Improvement of specific growth rate of Pichia pastoris for effective porcine interferon-α production with an on-line model-based glycerol feeding strategy

@article{Gao2011ImprovementOS,
  title={Improvement of specific growth rate of Pichia pastoris for effective porcine interferon-α production with an on-line model-based glycerol feeding strategy},
  author={Min-Jie Gao and Zhi-yong Zheng and Jian-Rong Wu and Shi-juan Dong and Zhen Li and H R Jin and Xiaobei Zhan and Chi-Chung Lin},
  journal={Applied Microbiology and Biotechnology},
  year={2011},
  volume={93},
  pages={1437-1445}
}
Effective expression of porcine interferon-α (pIFN-α) with recombinant Pichia pastoris was conducted in a bench-scale fermentor. The influence of the glycerol feeding strategy on the specific growth rate and protein production was investigated. The traditional DO-stat feeding strategy led to very low cell growth rate resulting in low dry cell weight (DCW) of about 90 g/L during the subsequent induction phase. The previously reported Artificial Neural Network Pattern Recognition (ANNPR) model… CONTINUE READING
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