Improvement of sensitivity in HLA-DRB1 typing by semi-nested PCR-RFLP.

  title={Improvement of sensitivity in HLA-DRB1 typing by semi-nested PCR-RFLP.},
  author={Sanae Inoue and Yuji Yamamoto and Osamu Okamoto and Haruki Murakami and Satoru Miyaishi and Hideo Ishizu},
  journal={Acta medica Okayama},
  volume={52 6},
A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB generic primer pairs and the second round of PCRs (20 cycles) were performed using DRB1 group-specific primers. The products of the second round PCRs were digested with restriction endonucleases for the… CONTINUE READING