Improvement of regeneration and transformation systems for Cyclamen persicum using somatic embryo culture

  title={Improvement of regeneration and transformation systems for Cyclamen persicum using somatic embryo culture},
  author={Teruhiko Terakawa and Tomomichi Yamamura and Toshio Murayama},
  journal={Plant Biotechnology},
Cyclamen persicum is one of the most important pot plants in the world. We developed an effective method for somatic embryo-mediated plant regeneration and genetic transformation using Agrobacterium, with somatic embryos as the source of plant material. Numerous somatic embryos were formed from the leaf segments of Cyclamen cv. "Fragrance Mini" 16 weeks after initiation on a 2,4-D- and kinetin-containing medium. The maximum regeneration frequency from the somatic embryos was achieved using a… 

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  • Plant Cell Tiss Org Cult
  • 2002