Improvement of recombinant protein yield by a combination of transcriptional amplification and stabilization of gene expression.

@article{Cebolla2002ImprovementOR,
  title={Improvement of recombinant protein yield by a combination of transcriptional amplification and stabilization of gene expression.},
  author={{\'A}ngel Cebolla and Jose Luis Royo and V{\'i}ctor de Lorenzo and Eduardo Santero},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 10},
  pages={5034-41}
}
We explored the use of a cascade circuit for heterologous gene expression that consists of a regulatory module with a salicylate-inducible system that controls the expression of a second regulator, xylS2, whose product is activated by common inducers. Activation and increasing the concentration of the second regulator synergistically induced heterologous genes downstream of the Pm promoter in the expression module. This module can be placed in multicopy vectors or in the chromosome of a host… CONTINUE READING