Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria.

  title={Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria.},
  author={Nad{\`e}ge Philippe and J. Argente Alcaraz and Evelyne Coursange and Johannes Geiselmann and Dominique Schneider},
  volume={51 3},
Allelic exchange experiments allow investigation of the functions of many unknown genes identified during the sequencing of entire genomes. Isogenic strains differing by only specific mutations can be constructed. Among other tools, suicide plasmids are widely used for this task. They present many advantages because they leave no scars on the chromosome, and therefore allow combining several mutations in the same genetic background. While using the previously described pCVD442 suicide plasmid… CONTINUE READING
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