Improvement of human interferon alpha secretion by Lactococcus lactis

@article{Zhang2010ImprovementOH,
  title={Improvement of human interferon alpha secretion by Lactococcus lactis},
  author={Qiuxiang Zhang and Jin Zhong and Xiaobo Liang and Wenjun Liu and Liandong Huan},
  journal={Biotechnology Letters},
  year={2010},
  volume={32},
  pages={1271-1277}
}
To improve the secretion and expression of human interferon alpha 2b (IFN) in Lactococcus lactis, a synthetic pro-peptide, LEISSTCDA (LEISS), was fused to the N-terminus of IFN. This gave a higher secretion efficiency (12% vs. 5%) and yield (~2.8-fold) of IFN. The signal peptide, SPSlpA (SlpA, an S-layer protein of Lactobacillus brevis), was also tested to secrete IFN instead of SPUsp45 (Usp45, the main secreted protein in L. lactis). This gave increased IFN secretion (~3-fold) but lower total… CONTINUE READING

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