Improvement of gene transfer to cervical cancer cell lines using non-viral agents.

@article{Cho2001ImprovementOG,
  title={Improvement of gene transfer to cervical cancer cell lines using non-viral agents.},
  author={C. Cho and Y. Cho and B. Kang and J. S. Hwang and S. Park and D. Yoon},
  journal={Cancer letters},
  year={2001},
  volume={162 1},
  pages={
          75-85
        }
}
Virus-like particles (VLPs) composed of recombinant capsid protein L1 and L2 of human papillomavirus type 16 were conjugated with polylysine (PL) and gene transfer was performed using VLP-PL conjugates to allow the expression of targeted gene. When HeLa cells were incubated with VLP-PL conjugate coupled with plasmid cytomegalovirus beta-galactosidase (pCMVbeta-gal), about 10% of cells were transfected and demonstrated beta-galactosidase activity. Hence chloramphenicol acetyltransferase activity… Expand
Production and Prophylactic Efficacy Study of Human Papillomavirus-like Particle Expressing HPV16 L1 Capsid Protein
TLDR
The neutralization activity of antibodies in the sera from the vaccinated mice was demonstrated by a rapid and simple assay to detect hemagglutihation inhibition activity and the vaccine efficacy of the purified HPV16 VLPs was validated and ELISA assay was performed. Expand
Development of a 293T Cell Line for the Production of Virus Like Particles in Bioreactors
Molecular therapy has become increasingly important for the treatment of some diseases. Although several protocols are currently in evaluation using viral and non viral delivery vectors, safetyExpand
Transcriptional activation of gene expression by pluronic block copolymers in stably and transiently transfected cells.
TLDR
Results indicate that Pluronics can increase the transcription of genes, in part, through the activation of selected stress signaling pathways through the Activation of the inflammation signaling pathway in myoblast cells by Pluronic. Expand
Nonviral vectors for gene delivery.
TLDR
Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells. Expand
Progress in developing cationic vectors for non-viral systemic gene therapy against cancer.
TLDR
The aim of this review is to describe the "perfect vector" for systemic gene therapy against cancer based on the advantages and disadvantages of existing vectors and on the hurdles encountered with these carriers. Expand
Follicle stimulating hormone receptor mediated gene delivery to target ovarian carcinoma in vitro
Background: How to deliver sufficient therapeutic genes into cancer cells while causing fewer side effects are major challenges in cancer gene therapies. Receptor mediated gene delivery enablesExpand
Follicle stimulating hormone receptor mediated gene delivery to target ovarian carcinoma in vitro
TLDR
The FSH-PEG-PEI conjugate can be used as a novel receptor mediated gene delivery system against ovarian carcinomas with improved biocompatibility in vitro and could be extended to a wide range of targeted gene therapeutics against different cancers. Expand
Emerging vectors and targeting methods for nonviral gene therapy
TLDR
Until recently, nonviral vectors were outside the mainstream of gene transfer technology, but recent problems in clinical trials using viral vectors renewed interest in these methods, and developments of new physical methods and various chemical carriers have been shown to achieve high-level gene delivery and functional expression. Expand
Gene recombinant bone marrow mesenchymal stem cells as a tumor-targeted suicide gene delivery vehicle in pulmonary metastasis therapy using non-viral transfection.
TLDR
In vitro results demonstrate that the engineered BMSCs have significant suicide effects in the presence of ganciclovir in a dose-dependent manner and can exert a sufficient bystander effect on B16F10 tumor cells in co-culture experiments, demonstrating clear therapeutic effects. Expand
Mesenchymal stem cells as a novel carrier for targeted delivery of gene in cancer therapy based on nonviral transfection.
TLDR
Results supported both the effectiveness of nonviral vectors in transferring the therapeutic gene to MSCs and the feasibility of using MSC’s as a targeted gene delivery carrier, indicating that M SCs could be a promising tumor target delivery vehicle in cancer gene therapy based onnonviral gene recombination. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 35 REFERENCES
Papillomavirus capsid binding and uptake by cells from different tissues and species.
TLDR
The results suggest that the PV receptor is a conserved cell surface molecule(s) used by different PV and that the tropism of infection byDifferent PV is controlled by events downstream of the initial binding and uptake. Expand
Expression of human papillomavirus type 11 L1 protein in insect cells: in vivo and in vitro assembly of viruslike particles
The L1 coat protein of human papillomavirus type 11 (HPV-11) was expressed in Sf-9 insect cells with the recombinant baculovirus vector Ac11L1. Viruslike particles (VLPs) were identified by electronExpand
In vitro gene transfer using human papillomavirus-like particles.
TLDR
It is reported here that human papillomavirus-like particles composed of the major capsid protein (L1) of HPV-16 are able to package unrelated plasmid DNA in vitro and then to deliver this foreign DNA to eukaryotic cells with the subsequent expression of the encoded gene. Expand
Expression of vaccinia recombinant HPV 16 L1 and L2 ORF proteins in epithelial cells is sufficient for assembly of HPV virion-like particles.
TLDR
The production of HPV-like particles using recombinant vaccinia virus should be useful for biochemical studies and could provide a safe source of material for the development of a vaccine. Expand
Efficient self-assembly of human papillomavirus type 16 L1 and L1-L2 into virus-like particles
TLDR
The ability to generate preparative amounts of HPV16 L1 and L1-L2 VLP may have implications for the development of a serological assay to detect anti-HPV16 virion immune responses to conformational epitopes and for immunoprophylaxis against HPV16 infection. Expand
Binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells
TLDR
Cell lines originating from a variety of tissues and organisms as distantly related as insects and humans bound VLPs with similar efficiency and specificity, suggesting that the putative receptor mediating VLP attachment should be highly conserved and cannot be responsible for the species and tissue specificity of HPVs. Expand
Identification of the alpha6 integrin as a candidate receptor for papillomaviruses
TLDR
Investigation of the interaction of human PV type 6b L1 virus-like particles (VLPs) with two epithelial cell lines, CV-1 and HaCaT, suggests that VLPs bind specifically to the alpha6 integrin subunit and that integrin complexes containing alpha6integrin complexed with either beta1 or beta4 integrins may act as a receptor for PV binding and entry into epithelial cells. Expand
Receptor-mediated gene delivery and expression in vivo.
  • G. Wu, C. Wu
  • Biology, Medicine
  • The Journal of biological chemistry
  • 1988
TLDR
A new soluble DNA carrier system can permit targeted delivery of foreign genes specifically to liver with resultant foreign gene expression in vivo through asialoglycoprotein receptors. Expand
Interaction of papillomaviruses with the cell surface
TLDR
The results suggest that papillomaviruses bind a widely expressed and evolutionarily conserved cell surface receptor. Expand
곤충세포에서 사람파필로마바이러스 16형 L1 재조합 단백질의 생산 및 바이러스 유사 입자분리
TLDR
The L1 capsid protein was expressed in Sf-21 insect cells via a baculovirus vector and could be purified in preparative amounts by CsC1 density gradient, ultracentrifugation indicating that L1 protein alone is sufficient for virus like particle assembly in insect cells. Expand
...
1
2
3
4
...