Improvement of embryonic stem cell line derivation efficiency with novel medium, glucose concentration, and epigenetic modifications.

Abstract

Although the first mouse embryonic stem (ES) cell lines were derived 2 decades ago, and standard protocols for ES cell derivation are widely used today, the technical difficulty of these protocols still pose a challenge for many investigators attempting to produce large numbers of ES cell lines, and are limited to only a few mouse strains. Recently, glucose… (More)
DOI: 10.1089/clo.2008.0053

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