Improvement of dibenzothiophene desulfurization activity by removing the gene overlap in the dsz operon.

Abstract

Dibenzothiophene (DBT) and its derivatives can be microbially desulfurized by Dsz enzymes. We investigated the expressional characteristics of the dsz operon. The result revealed that the ratio of mRNA quantity of dszA, dszB, and dszC was 11:3.3:1; however, western blot analysis indicated that the expression level of dszB is far lower than that of dszC. Gene analysis revealed that the termination codon of dszA and the initiation codon of dszB overlapped, whereas there was a 13-bp gap between dszB and dszC. In order to get a better, steady expression of DszB, we removed this structure by overlap polymerase chain reaction (PCR) and expressed the redesigned dsz operon in Rhodococcus erythropolis. The desulfurization activity of resting cells prepared from R. erythropolis DR-2, which held the redesigned dsz operon, was about five-fold higher than that of R. erythropolis DR-1, which held the original dsz operon.

01002003002008200920102011201220132014201520162017
Citations per Year

199 Citations

Semantic Scholar estimates that this publication has 199 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Li2007ImprovementOD, title={Improvement of dibenzothiophene desulfurization activity by removing the gene overlap in the dsz operon.}, author={Guo-Qiang Li and Ting Ma and Shan-Shan Li and Hong Li and Feng-Lai Liang and Ru-Lin Liu}, journal={Bioscience, biotechnology, and biochemistry}, year={2007}, volume={71 4}, pages={849-54} }