Improvement of cellulase activity using error-prone rolling circle amplification and site-directed mutagenesis.

@article{Vu2012ImprovementOC,
  title={Improvement of cellulase activity using error-prone rolling circle amplification and site-directed mutagenesis.},
  author={Van Hanh Vu and Keun Hee Kim},
  journal={Journal of microbiology and biotechnology},
  year={2012},
  volume={22 5},
  pages={607-13}
}
Improvement of endoglucanase activity was accomplished by utilizing error-prone rolling circle amplification, supplemented with 1.7 mM MnCl2. This procedure generated random mutations in the Bacillus amyloliquefaciens endoglucanase gene with a frequency of 10 mutations per kilobase. Six mutated endoglucanase genes, recovered from six colonies, possessed endoglucanase activity between 2.50- and 3.12-folds higher than wild type. We sequenced these mutants, and the different mutated sites of… CONTINUE READING