Improvement of carcinogen detection in the BALB/3T3 cell transformation assay by using a rich basal medium supplemented with low concentration of serum and some growth factors.

@article{Kajiwara1997ImprovementOC,
  title={Improvement of carcinogen detection in the BALB/3T3 cell transformation assay by using a rich basal medium supplemented with low concentration of serum and some growth factors.},
  author={Yoshitsugu Kajiwara and Salem Ajimi and Atsuko Hosokawa and Keiko Maekawa},
  journal={Mutation research},
  year={1997},
  volume={393 1-2},
  pages={81-90}
}
To improve the detection sensitivity and reproducibility of the transformation assay using BALB/3T3 cells, we scrutinized a new assay method in which the cells were replated in a medium containing a low concentration of serum after carcinogen treatment. Dulbecco's modified Eagle's medium plus Ham F12 (DME.F12) supplemented with a mixture of insulin, transferrin, ethanolamine and sodium selenite (ITES) and a low concentration of fetal calf serum (FCS) caused transformation at a high frequency… CONTINUE READING