Improvement of a phiC31 integrase-based gene delivery system that confers high and continuous transgene expression.

@article{Watanabe2011ImprovementOA,
  title={Improvement of a phiC31 integrase-based gene delivery system that confers high and continuous transgene expression.},
  author={Satoshi Watanabe and Shingo Nakamura and Takayuki Sakurai and Koji Akasaka and Masahiro Sato},
  journal={New biotechnology},
  year={2011},
  volume={28 4},
  pages={312-9}
}
phiC31 integrase-based gene delivery has been developed. However, the expression of integrated transgenes is often suppressed by a negative position effect. To improve this system, we constructed a new phiC31 integrase-based expression vector that contains attB, an expression unit placed in reverse orientation with two sea urchin-derived Ars-insulators to avoid position effects. In vitro and in vivo transfection experiments revealed that this new system produces higher levels of transgene… CONTINUE READING