Improvement of a low pH antigen-antibody dissociation procedure for ELISA measurement of circulating anti-Aβ antibodies

@article{Li2006ImprovementOA,
  title={Improvement of a low pH antigen-antibody dissociation procedure for ELISA measurement of circulating anti-Aβ antibodies},
  author={Qingyou Li and Marcia N Gordon and Chuanhai Cao and Kenneth E Ugen and David Morgan},
  journal={BMC Neuroscience},
  year={2006},
  volume={8},
  pages={22 - 22}
}
Prior work from our group found that acid dissociation (pH 2.5 incubation) of serum from APP transgenic mice vaccinated against Aβ increased the apparent anti-Aβ titers, suggesting antibody masking by antigen in the ELISA assay. Subsequently, we found that pH 2.5 incubation of serum from unvaccinated non-transgenic mice showed antibody binding to Aβ1–42, but no increase when other proteins, including shorter Aβ peptides, coated the ELISA plate. To investigate further the effects of low pH… CONTINUE READING
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