Improvement of XTT assay performance for studies involving Candida albicans biofilms.

@article{Silva2008ImprovementOX,
  title={Improvement of XTT assay performance for studies involving Candida albicans biofilms.},
  author={Wander Jos{\'e} da Silva and Jayampath Seneviratne and Nipuna B Parahitiyawa and Edvaldo Ant{\^o}nio Ribeiro Rosa and Lakshman Perera Samaranayake and Altair Antoninha Del Bel Cury},
  journal={Brazilian dental journal},
  year={2008},
  volume={19 4},
  pages={364-9}
}
2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay has been used to study Candida biofilm formation. However, considering that the XTT reduction assay is dependent on cell activity, its use for evaluating mature biofilms may lead to inaccuracies since biofilm bottom cell layers tend to be relatively quiescent at later stages of biofilm formation. The aim of this study was to improve XTT reduction assay by adding glucose… CONTINUE READING

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