Improvement of Mitochondria Extract from Saccharomyces cerevisiae Characterization in Shotgun Proteomics Using Sheathless Capillary Electrophoresis Coupled to Tandem Mass Spectrometry.

@article{Ibrahim2016ImprovementOM,
  title={Improvement of Mitochondria Extract from Saccharomyces cerevisiae Characterization in Shotgun Proteomics Using Sheathless Capillary Electrophoresis Coupled to Tandem Mass Spectrometry.},
  author={Marianne Ibrahim and Rabah Gahoual and Ludovic Enkler and Hubert Becker and Johana Chicher and Philippe Hammann and Yannis-Nicolas François and Lauriane Kuhn and Emmanuelle Leize-Wagner},
  journal={Journal of chromatographic science},
  year={2016},
  volume={54 4},
  pages={653-63}
}
In this work, we describe the characterization of a quantity-limited sample (100 ng) of yeast mitochondria by shotgun bottom-up proteomics. Sample characterization was carried out by sheathless capillary electrophoresis, equipped with a high sensitivity porous tip and coupled to tandem mass spectrometry (CESI-MS-MS) and concomitantly with a state-of-art nano flow liquid chromatography coupled to a similar mass spectrometry (MS) system (nanoLC-MS-MS). With single injections, both nanoLC-MS-MS… CONTINUE READING