Improvement of H2O2 stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase.

@article{MiyazakiImamura2003ImprovementOH,
  title={Improvement of H2O2 stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase.},
  author={Chie Miyazaki-Imamura and Kazuyo Oohira and Ritsuko Kitagawa and Hideo Nakano and Tsuneo Yamane and Haruo Takahashi},
  journal={Protein engineering},
  year={2003},
  volume={16 6},
  pages={423-8}
}
A functional expression system for a heme protein of Phanerochaete chrysosporium, manganese peroxidase (MnP), was developed using the Escherichia coli in vitro coupled transcription/translation system in the presence of hemin and fungal protein disulfide isomerase. This system has allowed the high-throughput construction and screening of a large diversity of mutant heme enzymes and has made it possible to improve the enzymatic function efficiently. Here we increased the H2O2 stability of MnP… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 17 extracted citations

Similar Papers

Loading similar papers…