Improvement of Fibrinolytic Activity of Bacillus subtilis 168 by Integration of a Fibrinolytic Gene into the Chromosome.

@article{Jeong2015ImprovementOF,
  title={Improvement of Fibrinolytic Activity of Bacillus subtilis 168 by Integration of a Fibrinolytic Gene into the Chromosome.},
  author={Seon-Ju Jeong and Ji Yeong Park and Jae Yong Lee and Kang-Wook Lee and Kye Man Cho and Gyoung Min Kim and Jung-Hye Shin and Jong-Sang Kim and Jeong Hwan Kim},
  journal={Journal of microbiology and biotechnology},
  year={2015},
  volume={25 11},
  pages={
          1863-70
        }
}
Fibrinolytic enzyme genes (aprE2, aprE176, and aprE179) were introduced into the Bacillus subtilis 168 chromosome without any antibiotic resistance gene. An integration vector, pDG1662, was used to deliver the genes into the amyE site of B. subtilis 168. Integrants, SJ3-5nc, SJ176nc, and SJ179nc, were obtained after two successive homologous recombinations. The integration of each fibrinolytic gene into the middle of the amyE site was confirmed by phenotypes (Amy(-), Spec(S)) and colony PCR… 
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