Improvement of Barley yellow dwarf virus-PAV detection in single aphids using a fluorescent real time RT-PCR.

@article{Fabre2003ImprovementOB,
  title={Improvement of Barley yellow dwarf virus-PAV detection in single aphids using a fluorescent real time RT-PCR.},
  author={Fr{\'e}d{\'e}ric Fabre and Christine Kervarrec and Lucie Mieuzet and G{\'e}rard Riault and Aude Vialatte and Emmanuel Jacquot},
  journal={Journal of virological methods},
  year={2003},
  volume={110 1},
  pages={51-60}
}
One of the major factors determining the incidence of Barley yellow dwarf virus (BYDV) on autumn-sown cereals is the viruliferous state of immigrant winged aphids. This variable is assessed routinely using the enzyme-linked immunosorbant assay (ELISA). However, the threshold for virus detection by ELISA can lead to false negative results for aphids carrying less than 10(6) particles. Although molecular detection techniques enabling the detection of lower virus quantities in samples are… CONTINUE READING
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