Improvement in volume estimation from confocal sections after image deconvolution.

  title={Improvement in volume estimation from confocal sections after image deconvolution.},
  author={Francesco Difato and Fabio Mazzone and Silvia Scaglione and Marco Fato and Francesco Beltrame and Lucie Kub{\'i}nov{\'a} and Jiri Janacek and Paola Ramoino and G. Vicidomini and Alberto Diaspro},
  journal={Microscopy research and technique},
  volume={64 2},
The confocal microscope can image a specimen in its natural environment forming a 3D image of the whole structure by scanning it and collecting light through a small aperture (pinhole), allowing in vivo and in vitro observations. So far, the confocal fluorescence microscope (CFM) is considered a true volume imager because of the role of the pinhole that rejects information coming from out-of-focus planes. Unfortunately, intrinsic imaging properties of the optical scheme presently employed yield… CONTINUE READING


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