Improvement in the resolution of o-phthalaldehyde derivatized amino acids by applying gradient steepness optimization to five reversed-phase columns of different lengths and particle sizes.

Abstract

Twenty-two o-phthalaldehyde (OPA) derivatized amino acids were separated on five different reversed-phase, octadecyl columns. Each column was packed with the same type of spherical silica on which matched bonding chemistry had been performed. Columns of 250, 150 or 50 mm x 4.5 mm I.D. were packed with 5 microns particles, and columns of 100 or 50 mm x 4.5… (More)

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