Improvement in the in vitro maturation rate of porcine oocytes vitrified at the germinal vesicle stage by treatment with a mitochondrial permeability transition inhibitor.

@article{Nakagawa2008ImprovementIT,
  title={Improvement in the in vitro maturation rate of porcine oocytes vitrified at the germinal vesicle stage by treatment with a mitochondrial permeability transition inhibitor.},
  author={Shoma Nakagawa and Akihiro Yoneda and Koji Hayakawa and Tomomasa Watanabe},
  journal={Cryobiology},
  year={2008},
  volume={57 3},
  pages={269-75}
}
In this study, we examined the effects of inhibitors of mitochondrial permeability transition (MPT), caspase activity, intracellular Ca(2+) chelator and mitochondrial Ca(2+) uniporter on survival assessed by morphological observation and in vitro maturation (IVM) of porcine vitrified germinal vesicle (GV) oocytes. When vitrified GV oocytes were matured only present in the IVM medium with an MPT inhibitor, cyclosporin A (CsA), the survival and IVM rates (36.1% and 26.8%, respectively) were… CONTINUE READING