Improvement in the high-performance liquid chromatography malondialdehyde level determination in normal human plasma.

@article{Volpi1998ImprovementIT,
  title={Improvement in the high-performance liquid chromatography malondialdehyde level determination in normal human plasma.},
  author={Nicola Volpi and Patriaia Tarugi},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1998},
  volume={713 2},
  pages={433-7}
}
We report a very rapid and simple isocratic reversed-phase HPLC separation of malondialdehyde (MDA) in normal human plasma without previous purification of the MDA-2-thiobarbituric acid (TBA) complex. The separation of MDA-TBA complex was performed using a 250x4.6 mm Nucleosil-5C18 column with a mobile phase composed of 35% methanol and 65% 50 mM sodium phosphate buffer, pH 7.0. Samples of 50 microl (composed of 100 microl plasma mixed with 1.0 ml of 0.2% 2-thiobarbituric acid in 2 M sodium… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 15 extracted citations

Evaluation of antioxidant defense systems in H4IIE cells infected with a retroviral vector.

Toxicology in vitro : an international journal published in association with BIBRA • 2010
View 1 Excerpt

Similar Papers

Loading similar papers…