Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil.

@article{Poly2001ImprovementIT,
  title={Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil.},
  author={Franck Poly and Lucile Jocteur Monrozier and Ren{\'e} Bally},
  journal={Research in microbiology},
  year={2001},
  volume={152 1},
  pages={95-103}
}
Several specific primers for the nifH gene were tested with different pure telluric N2-fixing strains. A PolF/PolR primer set provided successful amplification of 19 representative N2-fixing strains. Three restriction enzymes, HaeIII, NdeII and MnlI, chosen for restriction fragment length polymorphism (RFLP) analyses, were the most discriminating for the… CONTINUE READING