Improvement in sensitivity of allele-specific PCR facilitates reliable noninvasive prenatal detection of cystic fibrosis.


BACKGROUND Cell-free fetal DNA circulating in maternal blood has potential as a safer alternative to invasive methods of prenatal testing for paternally inherited genetic alterations, such as cystic fibrosis (CF) mutations. METHODS We used allele-specific PCR to detect mutated CF D1152H DNA in the presence of an excess of the corresponding wild-type… (More)

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