Improvement in recombinant protein production in ppGpp-deficient Escherichia coli.

@article{Dedhia1997ImprovementIR,
  title={Improvement in recombinant protein production in ppGpp-deficient Escherichia coli.},
  author={N N Dedhia and Rich Richins and A Mesina and Wilfred Chen},
  journal={Biotechnology and bioengineering},
  year={1997},
  volume={53 4},
  pages={379-86}
}
Maintaining a metabolically productive state for recombinant Escherichia coli remains a central problem for a wide variety of growth-dependent biosynthesis. This problem becomes particularly acute under conditions of minimal cell growth such as fed-batch fermentations. In this, we investigated the possibility of manipulating the protein synthesis machinery of E. coli whereby synthesis of foreign proteins might be decoupled from cell growth. In particular, the effects of eliminating… CONTINUE READING