Improvement and simplification of low‐background silver staining of proteins by using sodium dithionite

@article{Rabilloud1988ImprovementAS,
  title={Improvement and simplification of low‐background silver staining of proteins by using sodium dithionite},
  author={Thierry Rabilloud and G. Carpentier and Philippe Tarroux},
  journal={ELECTROPHORESIS},
  year={1988},
  volume={9}
}
High sensitivity and low background, the attractive characteristics of the procedure of Blum et al., Electrophoresis 1987, 8, 93–99, for silver staining of proteins in polyacrylamide gels have been improved by sensitizing the gels with sodium dithionite instead of sodium thiosulfate and by equilibration in water after fixation and prior to sensitization. These modifications decrease the background and allow for a longer development period, which in turn increases sensitivity and color contrast… 
A simplified silver diammine method for the staining of nucleic acids in polyacrylamide gels
High sensitivity and low background, which are the significant features of the procedure of Johansson and Skoog (J. Biochem. Biophys. Methods. 1987, 14, (Suppl.) 33) for silver staining of nucleic
A comparison between low background silver diammine and silver nitrate protein stains
TLDR
Among the long methods, those using glutaraldehyde treatment of the gel and silver diammine complex as the silvering agent were found to be the most sensitive, at the expense of the use of a modified polyacrylamide matrix and higher silver concentrations.
Differentiation of proteins in polyacrylamide gels by a modification of silver staining for the PhastSystem and a laser densitometer
TLDR
A modified silver staining method for the PhastSystem to reduce nonspecific background staining of the gel matrix leads to an improved detection of protein bands and virtually identical zero lines for the laser densitograms and the stain baselines.
A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate‐polyacrylamide gels
A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The
Silver-staining of proteins in polyacrylamide gels: a general overview.
TLDR
The most valuable protocols are presented in this report, including standard methods for unsupported gels and new methods devised for thin (0.5 mm) supported gels for SDS electrophoresis or isoelectric focusing and for staining of small peptides.
Modified silver staining for immobilized pH gradients
TLDR
A variant of silver staining in which thiosulfate is used twice, prior to silver impregnation and during development, at the micro‐molar level, to decrease the background.
Ultrasensitive protein detection and imaging: comparison of Lumitein™, ProteoSilver™, SYPRO(®) Ruby, and Coomassie (®) Brilliant Blue gel stains.
TLDR
The UVP BioImaging System in combination with LabWorks Image and Acquisition software is described to provide a comparison of four different protein gel stains and it is demonstrated that the detection sensitivity limit appears to be between 100 and 500 ng/protein band with Coomassie(®) Brilliant Blue.
Visible and fluorescent staining of two-dimensional gels.
TLDR
The present chapter describes procedures for several visible and fluorescent dyes compatible with mass spectrometry: colloidal Coomassie blue, silver nitrate, Sypro Ruby, Deep Purple, and 5-hexadecanoylamino-fluorescein.
Quantitation in two‐dimensional fluorescence difference gel electrophoresis: Effect of protein fixation
TLDR
It is concluded that when dealing with a large sample size with variable protein diffusion across the 2‐D gel over a period of 2–4 days, it is a preferred choice to fix the gel without affecting the protein quantitation.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 15 REFERENCES
Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining
The study of effects of several parameters on silver staining of proteins has led to the development of a staining method which is simple and reliable, requires only few stable solutions, and can be
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
A new modification of silver staining is presented which utilizes two chemical properties of thiosulfate: image enhancement by pretreatment of fixed gels, and formation of soluble silver complexes
A simplification of Heukeshoven and Dernick's silver staining of proteins
The procedure of Heukeshoven and Dernick for silver staining of proteins in polyacrylamide gels is simplified by applying Farmer's redücer before impregnation with silver nitrate and rinsing the gel
Silver staining of proteins in polyacrylamide gels.
Quantitative aspects of silver deposition in proteins resolved in complex polyacrylamide gels
Deposition of silver or silver compounds in proteins separated in linear gradient polyacrylamide electrophoretic gels is influenced by protein concentration and location, polyacrylamide
Quantitation of microgram amounts of protein in two‐dimensional polyacrylamide gel electrophoresis sample buffer
The concentration of protein actually solubilized in sample buffer in preparation for analysis by two‐dimensional polyacrylamide gel electrophoresis cannot be directly determined by the Lowry,
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
A new silver stain for electrophoretically separated polypeptides can be rapidly and easily used and can detect as little as 0.01 nanogram of protein per square millimeter. When employed with
...
1
2
...