Improved visualization of vertebrate nuclear pore complexes by field emission scanning electron microscopy.

@article{Shaulov2012ImprovedVO,
  title={Improved visualization of vertebrate nuclear pore complexes by field emission scanning electron microscopy.},
  author={Lihi Shaulov and Amnon Harel},
  journal={Structure},
  year={2012},
  volume={20 3},
  pages={
          407-13
        }
}
Field emission scanning electron microscopy (FESEM) can provide high-resolution three-dimensional surface imaging of many biological structures, including nuclear envelopes and nuclear pore complexes (NPCs). For this purpose, it is important to preserve NPCs as close as possible to their native morphology, embedded in undamaged nuclear membranes. We present optimized methodologies for FESEM imaging in a cell-free reconstitution system and for the direct visualization of mammalian cell nuclei… CONTINUE READING
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