Improved vectors and genome-wide libraries for CRISPR screening

@article{Sanjana2014ImprovedVA,
  title={Improved vectors and genome-wide libraries for CRISPR screening},
  author={Neville E. Sanjana and Ophir Shalem and Fengge Zhang},
  journal={Nature Methods},
  year={2014},
  volume={11},
  pages={783-784}
}
several modifications, including removal of one of the nuclear localization signals, human-codon optimization of the remaining nuclear localization signal and P2A bicistronic linker sequences, and repositioning of the U6-driven sgRNA cassette (Fig. 1a). These changes resulted in an approximately tenfold increase in functional viral titer over that of lentiCRISPRv1 (ref. 1; Fig. 1b). Improved vectors and genome-wide libraries for CRISPR screening 
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