Improved two-dimensional reversed phase-reversed phase LC-MS/MS approach for identification of peptide-protein interactions.

@article{Stephanowitz2012ImprovedTR,
  title={Improved two-dimensional reversed phase-reversed phase LC-MS/MS approach for identification of peptide-protein interactions.},
  author={Heike Stephanowitz and Sabine Lange and Diana Lang and Christian Freund and Eberhard Krause},
  journal={Journal of proteome research},
  year={2012},
  volume={11 2},
  pages={1175-83}
}
Quantitative mass spectrometry (MS) in combination with affinity purification approaches allows for an unbiased study of protein-protein and peptide-protein interactions. In shotgun approaches that are based on proteolytic digestion of complex protein mixtures followed by two-dimensional liquid-phase chromatography, the separation effort prior to MS analysis is focused on tryptic peptides. Here we developed an improved offline 2-D liquid chromatography-MS/MS approach for the identification and… CONTINUE READING