Improved transfer of two-dimensional crystals from the air/water interface to specimen support grids for high-resolution analysis by electron microscopy.

@article{Kubalek1991ImprovedTO,
  title={Improved transfer of two-dimensional crystals from the air/water interface to specimen support grids for high-resolution analysis by electron microscopy.},
  author={Elizabeth W. Kubalek and Roger D. Kornberg and Seth A Darst},
  journal={Ultramicroscopy},
  year={1991},
  volume={35 3-4},
  pages={295-304}
}
Electron crystallographic analysis of two-dimensional crystals grown on lipid layers at the air/water interface has been limited by loss or damage during transfer of the crystals to an electron microscope support grid. Two methods of transfer are described which are applicable on a small scale (10 microliters of protein solution) and which give greatly improved results for streptavidin crystals on biotinylated lipid layers. In the first method, a hydrophobic grid surface was produced by coating… CONTINUE READING