Improved template preparation for PCR-based assays for detection of food-borne bacterial pathogens.

@article{Lampel2000ImprovedTP,
  title={Improved template preparation for PCR-based assays for detection of food-borne bacterial pathogens.},
  author={Keith A. Lampel and Palmer A. Orlandi and Leroy Kornegay},
  journal={Applied and environmental microbiology},
  year={2000},
  volume={66 10},
  pages={4539-42}
}
Shigella flexneri, Salmonella enterica serotype Typhimurium, and Listeria monocytogenes were applied to FTA filters, and the filters were used directly as templates to demonstrate their sensitivity and applicability in PCR-based detection assays. With pure cultures, the sensitivities of detection by FTA filter-based PCR were 30 to 50 and 200 CFU for the gram-negative enterics and Listeria, respectively. Different numbers of S. flexneri cells were used in controlled contamination experiments… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.
36 Citations
22 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 36 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 22 references

Solid medium and method for DNA storage

  • L. A Burgoyne
  • 1996

Similar Papers

Loading similar papers…