Improved tandem affinity purification tag and methods for isolation of protein heterocomplexes from plants.

@article{Rohila2004ImprovedTA,
  title={Improved tandem affinity purification tag and methods for isolation of protein heterocomplexes from plants.},
  author={Jai S Rohila and Mei Chen and Ronald Cerny and Michael E. Fromm},
  journal={The Plant journal : for cell and molecular biology},
  year={2004},
  volume={38 1},
  pages={172-81}
}
A synthetic gene encoding the tandem affinity purification (TAP) tag has been constructed, and the TAP tag assayed for its effects on expression levels and subcellular localization by fusion to green fluorescent protein (GFP) as well as for its effects on steroid-dependent translocation to the nucleus and transcription when fused to a hybrid glucocorticoid receptor. A nuclear localization signal (NLS) was detected in the calmodulin-binding protein (CBP) domain and removed by mutation to improve… CONTINUE READING
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