Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes.

@article{Potgieter2009ImprovedSF,
  title={Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes.},
  author={Abraham Christiaan Potgieter and Nelson A. Page and Junita Liebenberg and Isabel M. Wright and Olfert Landt and Alberdina Aike van Dijk},
  journal={The Journal of general virology},
  year={2009},
  volume={90 Pt 6},
  pages={
          1423-32
        }
}
This paper reports significant improvements in the efficacy of sequence-independent amplification and quality of sequencing of viruses with segmented double-stranded RNA (dsRNA) genomes. We demonstrate that most remaining bottlenecks in dsRNA virus genome characterization have now been eliminated. Both the amplification and sequencing technologies used require no previous sequence knowledge of the viral dsRNA, there is no longer a need to separate genome segments or amplicons and the sequence… CONTINUE READING

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