Improved specificity of gene silencing by siRNAs containing unlocked nucleobase analogs

  title={Improved specificity of gene silencing by siRNAs containing unlocked nucleobase analogs},
  author={Narendra Vaish and Feng Chen and Shaguna Seth and Kathy Fosnaugh and Yan Liu and Roger C Adami and Tod Brown and Yan Chen and Pierrot Harvie and Rachel Johns and Gregory Severson and Brian Granger and Patrick Charmley and Michael E Houston and Michael V. Templin and Barry A. Polisky},
  booktitle={Nucleic acids research},
siRNAs confer sequence specific and robust silencing of mRNA. By virtue of these properties, siRNAs have become therapeutic candidates for disease intervention. However, their use as therapeutic agents can be hampered by unintended off-target effects by either or both strands of the siRNA duplex. We report here that unlocked nucleobase analogs (UNAs) confer desirable properties to siRNAs. Addition of a single UNA at the 5'-terminus of the passenger strand blocks participation of the passenger… CONTINUE READING
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