Improved solvent extraction procedure and high-performance liquid chromatography-evaporative light-scattering detector method for analysis of polar lipids from dairy materials.

@article{Le2011ImprovedSE,
  title={Improved solvent extraction procedure and high-performance liquid chromatography-evaporative light-scattering detector method for analysis of polar lipids from dairy materials.},
  author={Thien Trung Le and Jelena B. Miocinovic and Tuyet S. M. Nguyen and Roeland Rombaut and John Van Camp and Koen Dewettinck},
  journal={Journal of agricultural and food chemistry},
  year={2011},
  volume={59 19},
  pages={10407-13}
}
A normal-phase high-performance liquid chromatography-evaporative light-scattering detector method employing dichloromethane, methanol, and acetic acid/triethylamine buffer as the mobile phase was developed for analysis of polar lipids (PLs). This method was applicable for analysis of PLs from both dairy materials and soy lecithin. All of the PLs of interest such as glycolipids, phospholipids, and sphingomyelin were well separated with a total run time of 22.5 min and without necessitating the… CONTINUE READING

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