Improved radiolabeled substrates for soluble epoxide hydrolase.

@article{Borhan1995ImprovedRS,
  title={Improved radiolabeled substrates for soluble epoxide hydrolase.},
  author={Babak Borhan and Tesfamariam Mebrahtu and Saman Nazarian and Mark J Kurth and Bruce D Hammock},
  journal={Analytical biochemistry},
  year={1995},
  volume={231 1},
  pages={188-200}
}
Two rapid assays for the soluble epoxide hydrolase (sEH) are described. First, a sensitive radiometric assay based on thin-layer chromatography of [(14)C]-cis-9,10-epoxystearic acid and its corresponding diol ((14)C]-9,10-dihydroxystearic acid) is described. The cis fatty acid oxide exhibits higher specific activity of hydration with sEH from mouse, rat, human, and potato compared to trans-stilbene oxide (TSO). The K(m) and V(max) obtained for [(14)C]-cis-9,10-epoxystearic acid with mouse sEH… CONTINUE READING
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