Improved quantitative real‐time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard

@inproceedings{Coyne2005ImprovedQR,
  title={Improved quantitative real‐time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard},
  author={Kathryn J Coyne and Sara M. Handy and Elif Buğra Kuzu Demir and Edward B. Whereat and David A. Hutchins and Kevin J Portune and Martina A Doblin and Stephen C. Cary},
  year={2005}
}
Quantitative real-time PCR (QPCR) is a powerful and sensitive method for quantitative detection of microorganisms. Application of this methodology for enumeration of harmful algal bloom (HAB) species has the potential to revolutionize our approach to HAB research, making it possible to identify correlations between cell abundances and factors that regulate bloom dynamics. Its application to ecological studies, however, has produced mixed results. QPCR assays typically rely on the generation of… CONTINUE READING

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