Improved purification of the membrane-bound hydrogenase-sulfur-reductase complex from thermophilic archaea using epsilon-aminocaproic acid-containing chromatography buffers.

@article{Laska2000ImprovedPO,
  title={Improved purification of the membrane-bound hydrogenase-sulfur-reductase complex from thermophilic archaea using epsilon-aminocaproic acid-containing chromatography buffers.},
  author={S. Laska and A. Kletzin},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={2000},
  volume={737 1-2},
  pages={
          151-60
        }
}
  • S. Laska, A. Kletzin
  • Published 2000
  • Chemistry, Medicine
  • Journal of chromatography. B, Biomedical sciences and applications
A hydrogenase-sulfur reductase (SR) complex was purified from membrane preparations of the extremely thermophilic, acidophilic archaeon Acidianus ambivalens using a combination of sucrose density gradient centrifugation and column chromatography (FPLC). All chromatographic steps were performed in the presence of 0.5% epsilon-aminocaproic acid resulting in the elution of the SR complex as a sharp peak. In contrast, chromatography using buffers without epsilon-aminocaproic acid, or in the… Expand
Hydrogenases of the Model Hyperthermophiles
Archaeal 3′-phosphate RNA splicing ligase characterization identifies the missing component in tRNA maturation
Supramolecular organization in prokaryotic respiratory systems.

References

SHOWING 1-10 OF 21 REFERENCES
The quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes.
...
1
2
3
...