Improved purification of human bone sialoprotein and development of a homologous radioimmunoassay.

Abstract

Bone sialoprotein (BSP) is a phosphorylated skeletal glycoprotein. Here we describe a new procedure for the purification of BSP involving wide-pore reversed-phase HPLC, and the development of a homologous RIA for human BSP. The immunoassay showed linearity between 3 and 120 micrograms/L, a lower detection limit of 0.7 micrograms/L, and a mean recovery rate… (More)

Topics

Cite this paper

@article{Karmatschek1997ImprovedPO, title={Improved purification of human bone sialoprotein and development of a homologous radioimmunoassay.}, author={M Karmatschek and Irmgard Maier and M. J. Seibel and H. W. Woitge and Rudy Ziegler and Franz Paul Armbruster}, journal={Clinical chemistry}, year={1997}, volume={43 11}, pages={2076-82} }