Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system

@article{Ruottinen2008ImprovedPO,
  title={Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system},
  author={Maria Ruottinen and M{\'o}nika Boll{\'o}k and Martin K{\"o}gler and Antje Neubauer and Mirja Krause and Eija-Riitta H{\"a}m{\"a}l{\"a}inen and Johanna Myllyharju and Antti Vasala and Peter Neubauer},
  journal={BMC Biotechnology},
  year={2008},
  volume={8},
  pages={33 - 33}
}
BACKGROUND Here we describe a new technical solution for optimization of Pichia pastoris shake flask cultures with the example of production of stable human type II collagen. Production of recombinant proteins in P. pastoris is usually performed by controlling gene expression with the strong AOX1 promoter, which is induced by addition of methanol. Optimization of processes using the AOX1 promoter in P. pastoris is generally done in bioreactors by fed-batch fermentation with a controlled… CONTINUE READING
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