Improved production of heterologous protein from Streptomyces lividans

  title={Improved production of heterologous protein from Streptomyces lividans},
  author={Gregory F. Payne and Neslihan Delacruz and Steven J. Coppella},
  journal={Applied Microbiology and Biotechnology},
SummaryProtein-secreting procaryotic host organisms are currently being sought as alternatives to Escherichia coli for recombinant processing. In this study we examined how manipulation of the cultivation conditions can enhance heterologous protein production by Streptomyces lividans. The recombinant S. lividans used in this study expressed and excreted a Flavobacterium enzyme capable of hydrolyzing organophosphates. Initial shake-flask studies demonstrated that supplementing Luria-Bertani… Expand
Fermentation study for the production of hepatitis B virus pre-S2 antigen by the methylotrophic yeastHansenula polymorpha
Various physico-chemical parameters have been studied in order to improve the production of hepatitis B virus pre-S2 antigen by the methylotrophic yeastHansenula polymorpha, leading to a 140-fold increase in volumetric productivity of antigen and to a 4.6- fold increase in specific production. Expand
Native and heterologous protein secretion by Streptomyces lividans
Preliminary data on the processing of α-amylase, a native Streptomyces protein, showed much more rapid processing and secretion, but nevertheless still revealed cell-associated, signal-cleaved protein. Expand
Characterization and large-scale production of recombinant Streptoverticillium platensis transglutaminase
Enzyme activity was not affected by Ca2+, Li+, Mn2+, Na+, Fe3+, K+, Mg2+, Al3+, Ba2+, Co2+, EDTA, or IAA but was inhibited by Fe2+, Pb2+, Zn2+, Cu2+, Hg2+ PCMB, NEM, and PMSF. Expand
Monitoring transport of a recombinant phosphotriesterase in Streptomyces lividans using a pulse-chase system
A system to label the protein and follow its expression and secretion was developed and indicates a relatively slow rate of secretion that is also dependent on the growth medium. Expand
System-level understanding of gene expression and regulation for engineering secondary metabolite production in Streptomyces
The current status of the system-wide investigation of Streptomyces in terms of RNA is described, toward expansion of its genetic potential for secondary metabolite synthesis. Expand
Manipulating posttranslational modification in natural product biosynthesis
This dissertation focuses on evaluating the use of PPTase inhibitors to enable the extension of the chemoenzymatically installed 4'-PP arms to heterologously expressed synthase enzymes, and efforts to identify a new inhibitor scaffolds by the screening of large chemical libraries. Expand
Effects of Nonstarch Genetic Modifications on Starch Structure and Properties
It was found that the Cry1c transgenic lines showed a lower gelatinization temperature and faster digestion rate than the control or Bar lines, however, a slower digestion rate is nutritionally desirable. Expand
Iterative marker excision system
The establishment of an iterative marker excision system (IMES) based on the phiC31 integrase and its mutant att sites can be used for highly effective deletion of DNA fragments between inversely oriented B-CC and P-GG sites without risking unwanted DNA recombination. Expand
Evaluation of the feasibility of applying liquid-core capsules for in-situ product recovery of geldanamycin in a streptomyces hygroscopicus fermentation
Justine Burlet 20.02.2008 1/3 Evaluation of the feasibility of applying Liquid-Core capsules for In-Situ Product Recovery of Geldanamycin in a Streptomyces hygroscopicus fermentation Objective TheExpand
Investigating high-affinity non-covalent protein-ligand interaction via variants of streptavidin
Trapavidin has the potential to replace streptavidin in many of its diverse applications, as well as providing an insight into the nature of ultra-stable noncovalent interactions. Expand


Expression of a Thermomonospora fusca Cellulase Gene in Streptomyces lividans and Bacillus subtilis
  • G. Ghangas, David B. Wilson
  • Biology, Medicine
  • Applied and environmental microbiology
  • 1987
A cellulase gene from Thermomonospora fusca coding for endocellulase E(5) was introduced into Streptomyces lividans by using shuttle plasmids that can replicate in either S. lividan or Escherichia coli, selecting for thiostrepton resistance. Expand
Glucose metabolism and pyruvate excretion by streptomyces alboniger
Analysis of the metabolism of radiolabelled mannose and sucrose in the presence and absence of glucose demonstrated that glucose functions as the preferred carbon source, inhibiting the uptake and oxidation of the sugars within 15 min of its addition. Expand
Excretion of alpha-keto acids by strains of Streptomyces venezuelae.
Accumulation of pyruvic and alpha-ketoglutaric acids in S. venezuelae cultures grown in glucose-containing media may be due to regulatory suppression of the dehydrogenases by this carbon source. Expand
Development of recombinant Streptomyces for biotechnological and environmental uses.
Recent research that has confirmed that genetic exchange occurs readily among Streptomyces in the soil environment and which has shown the potential for exchange between recombinant StrePTomyces and native soil bacteria are summarized. Expand
Production of parathion hydrolase activity
SummaryA mixed bacterial culture which was obtained in a previous enrichment grew on parathion, an organophosphate insecticide, as a sole carbon and energy source. A cell-free enzyme preparation fromExpand
Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.
PIJ702 is a useful cloning vector with insertional inactivation of the Mel+ character as the basis of clone recognition and Restriction mapping of the tyrosinase fragment in pIJ702 revealed endonuclease cleavage sites for several enzymes, including single sites for BglII, SphI and SstI that are absent from the parent vector. Expand
Development of Streptomyces aureofaciens in submerged culture
During the biosynthetic production of chlortetracycline on a medium with sucrose, soy meal, corn steep extract, sodium chloride, ammonium sulphate, calcium carbonate and beet molasses, the amount ofExpand
Enzymatic Hydrolysis of Organophosphates: Cloning and Expression of a Parathion Hydrolase Gene from Pseudomonas diminuta
Pseudomonas diminuta strain MG hydrolyzes parathion to diethylthiophosphoric acid and p-nitrophenol and the esterase responsible for this reaction is encoded by a gene located on a plasmid termed pCMS1, which resulted in the isolation of transconjugants that exhibitedParathion hydrolase activity. Expand
Glucose-stimulated acidogenesis by Streptomyces peucetius.
Cultures of Streptomyces peucetius often were observed to divert spontaneously from the production of anthracycline antibiotics to the excretion of acidic metabolites, whether grown in a definedExpand
Expression of the xylanase gene of Streptomyces lividans and production of the enzyme on natural substrates.
Further studies on the expression of the xylanase gene in cultures on xylan and on some more economical lignocellulosic biomass are reported. Expand