Improved production of heterologous protein from Streptomyces lividans

@article{Payne2004ImprovedPO,
  title={Improved production of heterologous protein from Streptomyces lividans},
  author={Gregory F. Payne and Neslihan Delacruz and Steven J. Coppella},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={33},
  pages={395-400}
}
SummaryProtein-secreting procaryotic host organisms are currently being sought as alternatives to Escherichia coli for recombinant processing. In this study we examined how manipulation of the cultivation conditions can enhance heterologous protein production by Streptomyces lividans. The recombinant S. lividans used in this study expressed and excreted a Flavobacterium enzyme capable of hydrolyzing organophosphates. Initial shake-flask studies demonstrated that supplementing Luria-Bertani… Expand
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