Improved production of doubled haploids by colchicine application to wheat (Triticum aestivum L.) anther culture

@article{Redha1998ImprovedPO,
  title={Improved production of doubled haploids by colchicine application to wheat (Triticum aestivum L.) anther culture},
  author={Amina Redha and Tamer A Attia and B. B{\"u}ter and S. Saisingtong and Peter Stamp and Judith Elfriede Schmid},
  journal={Plant Cell Reports},
  year={1998},
  volume={17},
  pages={974-979}
}
The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100–5000 mg/l, was added to the induction medium for 1–5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductions in embryo–like structures as well as higher doubling indices were found. Similar results were obtained with the high- and low-responding genotypes. Colchicine (100 mg/l), added… CONTINUE READING
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