Improved preparation and assay and some characteristics of Cl--ATPase activity from Limonium vulgare.

@article{Auffret1981ImprovedPA,
  title={Improved preparation and assay and some characteristics of Cl--ATPase activity from Limonium vulgare.},
  author={C A Auffret and David E. Hanke},
  journal={Biochimica et biophysica acta},
  year={1981},
  volume={648 2},
  pages={186-91}
}
We present a revised method for the preparation, storage and assay of the Cl--ATPase activity responsible for salt secretion in Limonium vulgare. Altering the centrifugation step improved the yield and linked-enzyme assay for ADP provided a sensitive method for continuous monitoring of Cl--ATPase activity. The activity of untreated membranes was low but fairly stable. Treatment with detergent gave strong stimulation of Cl--ATPase activity ATP between 0.1 and 0.2 mM. Cl- stimulated the activity… CONTINUE READING