Improved poliovirus d-antigen yields by application of different Vero cell cultivation methods

@inproceedings{Thomassen2014ImprovedPD,
  title={Improved poliovirus d-antigen yields by application of different Vero cell cultivation methods},
  author={Yvonne E. Thomassen and Olaf Rubingh and Ren{\'e} H. Wijffels and Leo A van der Pol and Wilfried A. M. Bakker},
  booktitle={Vaccine},
  year={2014}
}
Vero cells were grown adherent to microcarriers (Cytodex 1; 3 g L(-1)) using animal component free media in stirred-tank type bioreactors. Different strategies for media refreshment, daily media replacement (semi-batch), continuous media replacement (perfusion) and recirculation of media, were compared with batch cultivation. Cell densities increased using a feed strategy from 1×10(6) cells mL(-1) during batch cultivation to 1.8, 2.7 and 5.0×10(6) cells mL(-1) during semi-batch, perfusion and… CONTINUE READING