Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication.

@article{Remaut1983ImprovedPV,
  title={Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication.},
  author={Erik Remaut and H Tsao and Walter Fiers},
  journal={Gene},
  year={1983},
  volume={22 1},
  pages={103-13}
}
Improved expression vectors have been constructed which are derived from runaway-replication mutants of plasmid R1 and carry the strong leftward promoter (pL) of bacteriophage lambda. The activity of this promoter is controlled by a temperature-sensitive repressor, product of the phage gene cI cloned on a compatible plasmid. Heat induction leads to amplification of the plasmid copy number and at the same time turns on the promoter. At a short distance downstream from the promoter, unique EcoRI… CONTINUE READING

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