Improved performance of enzyme-linked immunosorbent assays and the effect of human immunodeficiency virus coinfection on the serologic detection of herpes simplex virus type 2 in Rakai, Uganda.

@article{Gamiel2008ImprovedPO,
  title={Improved performance of enzyme-linked immunosorbent assays and the effect of human immunodeficiency virus coinfection on the serologic detection of herpes simplex virus type 2 in Rakai, Uganda.},
  author={Jordyn L. Gamiel and Aaron A. R. Tobian and Oliver Laeyendecker and Steven J. Reynolds and Rhoda L Ashley Morrow and David M Serwadda and Ronald Henry Gray and Thomas C Quinn},
  journal={Clinical and vaccine immunology : CVI},
  year={2008},
  volume={15 5},
  pages={888-90}
}
Ugandan subjects (820) were tested by Focus HerpeSelect enzyme-linked immunosorbent assay (ELISA), Kalon herpes simplex virus type 2 ELISA, and BioKit rapid test, and the results were compared to those of Western blotting. Higher-than-standard-index cutoff values gave optimal sensitivity and specificity. Kalon ELISA was the optimal assay when an index value of 1.5 was used (sensitivity, 91.7%; specificity, 92.4%). 
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