Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches

  title={Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches},
  author={Owen P. Hamill and Alain Marty and Erwin Neher and Bert J. Sakmann and Fred J. Sigworth},
  journal={Pfl{\"u}gers Archiv},
Abstract1.The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.2.A description of a convenient method for the fabrication of patch recording pipettes is given together with procedures followed to achieve giga-seals i.e. pipettemembrane seals with resistances of 109–1011Ω.3.The… Expand
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