Improved nonporous magnetic supports for immobilized enzymes.

  title={Improved nonporous magnetic supports for immobilized enzymes.},
  author={Peter James Halling and Peter Dunnill},
  journal={Biotechnology and bioengineering},
  volume={21 3},
Ni powders coated by deposition of TiO2 or controlled oxidation to NiO develop substantial resistance to corrosion. Chymotrypsin immobilized to these coated Ni supports shows very high stability of activity on storage. Chymotrypsin immobilized by adsorption and glutaraldehyde crosslinking was fairly rapidly eluted under operational conditions in the presence of substrate. If 3-aminopropyltriethoxysilane (APS) was used to produce a covalent linkage, desorption of enzyme still occurred because of… CONTINUE READING
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