Improved nematode extraction from carrot disk culture.

@article{Kaplan1990ImprovedNE,
  title={Improved nematode extraction from carrot disk culture.},
  author={David T. Kaplan and Eric L. Davis},
  journal={Journal of nematology},
  year={1990},
  volume={22 3},
  pages={399-406}
}
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes and eggs was attained when carrot tissue infested with Radopholus citrophilus or R. similis was macerated with a mixture of 0.50% driselase and 0.50% cellulysin, w/v each, with 2.5 ml of enzyme… CONTINUE READING
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