Improved negative selection protocol for Plasmodium berghei in the rodent malarial model

@inproceedings{Orr2012ImprovedNS,
  title={Improved negative selection protocol for Plasmodium berghei in the rodent malarial model},
  author={Rachael Y Orr and Nisha Philip and Andrew P Waters},
  booktitle={Malaria Journal},
  year={2012}
}
An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluorocytosine (5FC) by intraperitoneal injection for administration via the drinking water of the mice. The improved methodology is shown to be as effective, less labour-intensive, reduces animal handling and… CONTINUE READING
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